Yuav ua li cas xaiv cov raj PCR zoo?
Teb: BKMAM PCR hlab yog tsim rau cov khoom siv PCR tseem ceeb thiab cov cuab yeej PCR tiag tiag hauv kev ua lag luam (BD, Beckman thiab Wanshi PCR instruments).
Kev sib piv ntawm cov PCR ntawm lub sijhawm tiag tiag, digital PCR thiab ib txwm siv PCR:
Txheej txheem cej luam ntawm digital PCR: ntsuas qhov kev faib ua feem ntawm qhov tsis zoo replicates los txiav txim siab tus lej luam.
Txheej txheem cej luam ntawm Real-Time PCR: Kev ntsuas tau ua raws li PCR amplification tshwm sim.
Txheej txheem cej luam ntawm ib txwm PCR: ntsuas tus nqi ntawm cov khoom PCR khaws tseg thaum kawg ntawm lub voj voog PCR.
Puas yog digital PCR quantitative?
A: Yog lawm, qhov kev faib ua feem ntawm cov lus teb tsis zoo RCR haum rau Poisson statistical algorithm.
Puas yog PCR lub sijhawm tiag tiag?
A: Yog lawm, vim tias cov ntaub ntawv raug sau thaum lub sijhawm exponential (logarithmic) ntawm PCR, thaum lub sijhawm cov khoom PCR yog proportional rau tus nqi ntawm nucleic acid template.
Puas yog PCR ntau npaum li cas?
A: Tsis yog, tab sis kev sib piv qhov kev siv ntawm lub suab nrov ntawm cov gel mus rau tus qauv ntawm kev paub txog cov ntsiab lus tuaj yeem ua rau koj "semi-quantitative".
Daim ntawv thov ntawm cov PCR: qhov tseeb kom muaj nuj nqis ntawm cov kab mob kis, qhov tseeb ntawm cov qauv nucleic acid, meej kom muaj nuj nqis ntawm cov tsev qiv ntawv tiam tom ntej sequencing, nrhiav pom cov tsis tshua muaj alleles, meej kom muaj nuj nqis ntawm cov noob qhia, sib xyaw kom zoo thiab sib cais.
Daim ntawv thov ntawm PCR lub sij hawm tiag tiag: gene nthuav qhia kom muaj nuj nqis, microarray validation, zoo tswj thiab soj ntsuam validation, pathogen nrhiav
, SNP genotyping, luam tus lej variation, microRNA tsom xam, virus quantification, siRNA/RNAi thwmsim
Kev siv ntawm PCR ib txwm muaj: DNA amplification, sequencing, genotyping, molecular cloning
Xaus: Qhov zoo ntawm PCR digital: tsis tas yuav tso siab rau cov ntaub ntawv los yog cov qauv, kev xav tau precision tuaj yeem ua tiav los ntawm kev nce tag nrho cov PCR replicates, siab kam rau siab rau inhibitors, muaj peev xwm txheeb xyuas cov kev sib xyaw ua ke.
Tsis zoo li ib txwm qPCR, digital PCR teb linearly rau cov ntawv luam uas tshwm sim thiab qhov sib txawv me me tuaj yeem kuaj pom.
Qhov zoo ntawm PCR lub sijhawm tiag tiag: nce ntau qhov kev kuaj pom, tsis muaj kev ua haujlwm tom qab PCR yuav tsum tau, kev tshawb pom muaj peev xwm npog cov kev hloov pauv qis li 2-fold, cov ntaub ntawv tau txais thaum lub sij hawm exponential ntawm PCR, nce hauv cov xov xwm fluorescence teeb liab nrog Qhov tshwm sim amplicons Proportional nyob rau hauv tus lej, cleavage probes muab cov ntaub ntawv mus tas li ntawm cleavage ntawm amplicons
Qhov tsis zoo ntawm PCR ib txwm muaj: qhov tseeb tsis zoo, tsis tshua muaj siab, me me dynamic ntau <2 lub cav, kev daws teeb meem tsawg, tsis siv neeg, tsuas yog kev tshuaj xyuas qhov sib txawv raws li cov lus qhia tshwj xeeb, cov txiaj ntsig tsis sawv cev raws li tus lej, tsis txaus siv ethidium bromide staining, Post-PCR
Yog xav paub ntxiv txog PCR strip raj, thov hu rau peb txhua lub sijhawm:
Michael Peng
Changde BKMAM Biotechnology Co., Ltd
Chaw Nyob: Lub Tsev 1, East Of New Material Industrial Zone,
Linli Economic Development Zone, Changde, 415200 Hunan
Tel: plus 86-17363688083
Mob: plus 8613450479355(whatsapp&wechat)
Skype: peng.michael2011
https://hnbikeman.en.alibaba.com
Email:michael@bkmbio.com
One-stop laboratory consumables tsum!
Peb tab tom nrhiav rau cov neeg ua haujlwm ncaj ncees thoob plaws lub ntiaj teb thiab tos ntsoov rau kev koom tes nrog koj.