Kev taw qhia thiab kev sib piv ntawm txoj kev ua kom tsis muaj menyuam

Kev taw qhia thiab kev sib piv ntawm txoj kev ua kom tsis muaj menyuam

Nws hais txog kev tua tag nrho cov kab mob, nrog rau cov kab mob thiab cov kab mob uas tsis yog kab mob thiab cov kab mob, los ntawm kev siv lub cev lossis tshuaj lom neeg los xyuas kom tsis muaj menyuam. Cov khoom tsis muaj kab mob tom qab ua kom tsis muaj menyuam yog hu ua cov khoom tsis muaj menyuam. Qhov chaw tsis muaj kab mob tom qab ua kom tsis muaj menyuam yog hu ua aseptic area.

Txawm li cas los xij, rau ib pawg ntawm cov khoom siv kom tsis muaj menyuam, qhov tsis muaj menyuam tsis tuaj yeem tsis tuaj yeem lav lossis txheeb xyuas los ntawm kev sim. Kev ntsuas lub cev lossis tshuaj lom neeg ua kom tsis muaj menyuam qhia tau tias kev tua cov kab mob ua raws li txoj cai logarithmic. Yog li ntawd, tus qauv sterility ntawm sterilized khoom yog feem ntau qhia los ntawm qhov tshwm sim ntawm microbial ciaj sia taus tom qab sterilization - sterility assurance qib SAL.

Qee hom kev ua kom tsis muaj menyuam: qhuav cua sov kom tsis muaj menyuam, ntub dej kub kom tsis muaj menyuam, irradiation sterilization, tshuaj sterilization, pom sterilization, thiab lwm yam.

Moist kub sterilization

Txoj kev moist cua sov sterilization siv qhov kub thiab txias siab chav ua qhov nruab nrab. Vim qhov loj latent cua sov thiab muaj zog nkag mus ntawm chav, nws yog ib qho yooj yim rau denature los yog solidify proteins thiab nws thiaj li ua rau tuag ntawm cov kab mob. Yog li ntawd, txoj kev ua kom tsis muaj menyuam ntawm txoj kev no yog siab dua li ntawm txoj kev ua kom tsis muaj cua sov qhuav, uas yog txoj kev siv tshuaj tua kab ntau tshaj plaws hauv kev tsim cov tshuaj npaj. Cov cua sov kom tsis muaj menyuam feem ntau yog 121 degree × 20min. Thaum qhov kub thiab txias tau nce, lub sij hawm yuav raug txo kom haum. Feem ntau hais lus, qhov kub siab tshaj plaws tuaj yeem ncav cuag 134 degree. Lub sijhawm no, kev ua kom tsis muaj menyuam tuaj yeem siv sijhawm li ob peb feeb.

Rau cov khoom siv hauv cov chaw sim tshuaj lom neeg, feem ntau ntawm cov iav tuaj yeem tiv taus qhov kub ntawm cov cua sov kom tsis muaj menyuam, thaum cov khoom yas yuav tsum pom cov khoom sib txawv. Cov ntaub ntawv uas tuaj yeem ua kom tsis muaj menyuam los ntawm cov cua sov feem ntau muaj xws li PP, PPCO, PC thiab fluorinated polymer plastics. Lub chaw soj nstuam feem ntau siv qee cov khoom qhia los txiav txim seb qhov kub-kub thiab siab-siab sterilization lauj kaub ua tau raws li qhov teev tseg kom tsis muaj menyuam. Piv txwv li, daim kab xev qhia lossis daim npav qhia hauv daim duab hauv qab no thiab lwm yam khoom raug muab tso rau hauv lub lauj kaub kom tsis muaj menyuam ua ke nrog cov qauv kom tsis muaj menyuam. Yog tias qhov kev ua kom tsis muaj menyuam hauv lub lauj kaub tsim nyog, kev hloov pauv yuav tshwm sim, feem ntau los ntawm qhov ntiav mus rau qhov tob.

Nws yog ib qho tsim nyog sau cia tias cov khoom zoo li no tsuas tuaj yeem ua pov thawj tias kev ua kom tsis muaj menyuam hauv lub lauj kaub kom tsis muaj menyuam tau ntsib, tab sis tsis tuaj yeem ua pov thawj tias cov qauv ua kom tsis muaj menyuam tau mus txog lub xeev tsis muaj menyuam.

Qhuav cua sov kom tsis muaj menyuam

Qhuav cua sov kom tsis muaj menyuam yog ib qho cuab yeej los tua cov kab mob thiab cov kab mob spores nrog kub kub hauv qhov chaw qhuav. Nws yog siv rau cov khoom uas tsis tuaj yeem tiv taus qhov kub thiab av noo thiab tsis tuaj yeem ua kom tsis muaj zog nrog lub zog siab, xws li cov tshuaj uas yuav tsum tau khaws cia kom qhuav, cov cuab yeej ntse xws li riam thiab txiab, thiab cov roj tsis muaj dej, tshuaj pleev, glycerin, thiab lwm yam.

Qhuav cua sov kom tsis muaj menyuam yuav tsum tau kub dua thiab siv sij hawm ntev dua. Qhov kub ntau yog 160 degree, kav ntev li 2h. Cov cua sov qhuav yog qhov tsim nyog rau kev ua kom tsis muaj menyuam ntawm cov iav, tshwj xeeb tshaj yog iav koob txhaj tshuaj. Qhov kub tuaj yeem nce mus rau 170 degree thiab lub sijhawm ua kom tsis muaj menyuam yog 1h.

Thaum tshav kub kub qhuav yog teeb meem rau qee cov ntaub ntawv uas tiv taus cua sov thiab tsis haum rau daim ntawv thov. Txoj kev ziab tshav kub tsis tuaj yeem siv tsis tau rau cov khoom yas, vim tias txawm tias PP cov ntaub ntawv uas muaj qhov kub thiab txias tsuas tuaj yeem tiv taus 135 degree, thiab yuav luag tsuas yog fluorinated polymer plastics tuaj yeem tiv taus qhov kub thiab txias ib puag ncig ntawm cov cua sov kom tsis muaj menyuam.

Irradiation sterilization

Irradiation sterilization yog ib txoj hauv kev zoo los tua cov kab mob ntawm ntau yam khoom siv los ntawm kev siv hluav taws xob hluav taws xob uas tsim los ntawm ionizing hluav taws xob. Cov hluav taws xob siv rau kev ua kom tsis muaj menyuam muaj xws li hluav taws xob hluav taws xob, X-ray thiab Ray, thiab lwm yam. Lawv tuaj yeem tswj kev loj hlob ntawm cov kab mob los yog tua cov kab mob hauv ib txoj kev tshwj xeeb.

X-ray thiab Radiation tuaj yeem ua rau lwm yam khoom ua rau oxidize lossis tsim cov dawb radicals (OH · H) thiab tom qab ntawd ua rau cov roj ntsha molecules, lossis ncaj qha ua rau cov roj ntsha molecules, tawg hydrogen bonds, oxidize ob daim ntawv cog lus, rhuav tshem cov qauv ntawm lub nplhaib lossis polymerize qee cov molecules, thiab lwm yam, txhawm rau rhuav tshem thiab hloov cov qauv ntawm cov macromolecules lom, yog li inhibiting lossis tua cov kab mob.

Irradiation sterilization yog tsim rau cov khoom uas tsis tuaj yeem tiv taus cua sov los yog damp kub thiab kub ib puag ncig. Yog li ntawd, ntau yam khoom yas uas tsis tuaj yeem tiv taus qhov kub siab, xws li kab lis kev cai tais diav, kab lis kev cai ntawm tes, lub raj mis kab lis kev cai, yuav raug sterilized los ntawm irradiation.

Tshuaj sterilization

Tshuaj sterilization yog hais txog txoj kev tua cov kab mob los ntawm kev ua ncaj qha rau lawv nrog tshuaj. Cov tshuaj uas tuaj yeem tua tau cov kab mob hu ua germicides, uas tuaj yeem muab faib ua cov roj germicides thiab kua germicides. Tshuaj tua kab mob thiab ua kom tsis muaj menyuam tuaj yeem siv rau txhua yam khoom uas tsis haum rau kev tua kab mob hauv lub cev thiab ua kom tsis muaj menyuam. Ethylene oxide sterilization txoj kev yog qhia ntawm no.

Ethylene oxide yog ib qho roj, thiab ethylene oxide sterilization yog ib qho dav-spectrum, muaj txiaj ntsig, nkag mus rau kev ua kom tsis muaj menyuam nrog kev puas tsuaj me ntsis rau cov khoom siv tshuaj tua kab mob. Nws cov vapor siab yog qhov siab heev, thiab nws tuaj yeem nkag mus rau micropores kom ncav cuag qhov tob ntawm tsab xov xwm. Nws cov kua thiab roj muaj cov nyhuv bactericidal, thiab cov pa roj muaj zog bactericidal nyhuv. Ethylene oxide tuaj yeem tua tau ntau yam kab mob, nrog rau cov kab mob propagules, spores, fungi, kab mob, thiab lwm yam. Nws yog cov tshuaj tua kab mob dav dav.

Ethylene oxide sterilization muaj ntau heev hauv qee cov khoom siv kho mob thiab cov khoom siv kho mob. Txawm li cas los xij, nws yuav tsum tau muab sau tseg tias EO yog cov pa phem, yog li nws feem ntau yuav tsum tau qis dua ib qho concentration tom qab ua kom tsis muaj menyuam kom ua tau raws li qhov yuav tsum tau ua.

Lim sterilization

Kev lim dej tsis yog ib txoj kev ua kom tsis muaj menyuam. Txoj kev no yog ua raws li lub hauv paus ntsiab lus ntawm kev siv cov ntawv lim kom tshem tawm cov khoom particle impurities hauv cov kua, tshwj tsis yog tias qhov pore txoj kab uas hla ntawm lub lim membrane siv yog me me vim tias cov kab mob loj dua. Lub pore txoj kab uas hla ntawm feem ntau siv cov kab mob tshem tawm lim membrane ntawm lub khw yog {{0}}.22 μ m / 0.2 μm.

Txoj kev no tsuas yog siv tau rau kev ua kua thiab roj sterilization, piv txwv li, PES lim membrane feem ntau yog siv rau kab lis kev cai ntawm tes thiab ua kom tsis muaj menyuam; Thaum lub sij hawm kab lis kev cai ntawm tes, lub tshuab lim cua sterilizing txuas nrog lub qhov cua ntawm lub xov tooj ntawm tes kom ua tiav lub hom phiaj ntawm huab cua permeability thiab kom tsis muaj menyuam.

Pipette tswv yim, Centrifuge tubes, Cryogenic vials, Sampling hnab, Cov quav coj mus kuaj ntim ntim

Tag nrho peb cov khoom yog pheej yig heev raws li peb yog ib tug thawj chaw tsim tshuaj paus rau lab & kho mob consumables nyob rau hauv Tuam Tshoj.Koj kuj yuav tau siab chav sterilization qhia daim kab xev, syringe lim hauv BKMAM.