Nov yog qee qhov nruab nrab hauv kev sim
1. Khoom noj khoom haus agar kab lis kev cai nruab nrab
Khoom noj khoom haus agar kab lis kev cai nruab nrab yog siv los tsim thiab cog cov kab mob zoo tib yam rau tag nrho cov kab mob ntsuas, khaws cov kab mob thiab cov kab lis kev cai ntshiab. Cov khoom xyaw tseem ceeb yog: nqaij nyug immersion, poov xab extraction, protein, sodium chloride, agar hmoov, distilled dej. Protein thiab nqaij nyuj hmoov muab nitrogen, vitamins, amino acids, thiab carbon qhov chaw. Sodium chloride tuaj yeem tswj tau qhov sib npaug osmotic siab. Rau agar yog qhov nruab nrab ntawm qhov nruab nrab.
Nutritional agar yog hom nruab nrab tshaj plaws, uas muaj cov as-ham uas xav tau rau feem ntau cov kab mob kom loj hlob. Khoom noj khoom haus agar tuaj yeem siv tau thaum lub sij hawm cov kab mob kab mob.
2. Ntshav agar kab lis kev cai nruab nrab
Ntshav agar kab lis kev cai nruab nrab yog cov nqaij nyug muab tshuaj txhuam cov protein cashmere nruab nrab uas muaj cov dej dehydrated tsiaj (feem ntau cov ntshav luav lossis cov ntshav yaj). Yog li, ntxiv rau kev cog qoob loo ntau yam khoom noj uas xav tau rau cov kab mob, nws tuaj yeem muab cov coenzymes (xws li V factor), xws li V factor ). Kev loj hlob tshwj xeeb xws li heme (X factor). Yog li ntawd, cov ntshav nruab nrab feem ntau yog siv los cog, cais thiab khaws qee cov kab mob pathogenic uas yuav tsum tau noj zaub mov hnyav.
Tsis tas li ntawd, ntshav agar feem ntau yog siv rau kev kuaj hemolysis. Hauv cov txheej txheem ntawm kev loj hlob, qee cov kab mob tuaj yeem tsim hemolytin, uas tuaj yeem rhuav tshem cov qe ntshav liab thiab yaj. Thaum loj hlob ntawm cov ntsiav tshuaj phaj, pob tshab lossis pob tshab hemolytic nplhaib tuaj yeem pom nyob ib puag ncig cov cheeb tsam. Lub pathogenicity ntawm ntau cov kab mob muaj feem xyuam rau cov yam ntxwv hemolytic. Vim qhov sib txawv ntawm hemolytin tsim los ntawm cov kab mob sib txawv, lub peev xwm hemolytic txawv, thiab qhov tshwm sim hemolytic ntawm platelets txawv. Yog li ntawd, kev kuaj hemolytic feem ntau yog siv los txheeb xyuas cov kab mob.
3. TCBS nruab nrab
TCBS yog sulfur sulfate citrate citric acid ntsev ntsev ntsev ntsev ntsev sucrose agarmerine. Rau kev xaiv cais ntawm cov kab mob pathogenic. Protein pupae, poov xab molicment nyob rau hauv nruab nrab raws li lub hauv paus as-ham los muab ntau yam kev loj hlob yam xws li nitrogen, carbon qhov chaw, thiab vitamins yuav tsum tau rau cov kab mob loj hlob; siab dua ntawm sodium chloride kom tau raws li qhov xav tau ntawm kev loj hlob ntsev hauv cov ntsev ntawm lub pyramid; sucrose; sucrose; sucrose Raws li fermented carbon qhov chaw; Lub hav zoov ntawm sodium citrate, siab pH alkaline ib puag ncig thiab sodium sulfate inhibit qhov kev loj hlob ntawm cov kab mob hauv plab, thiab sodium gallbladder hmoov thiab sodium nqaij nyug gallate tsuas yog inhibit qhov kev loj hlob ntawm Gram-positive kab mob.
Tsis tas li ntawd, sodium sulfate kuj muab sulfur qaub sulfur. Nyob rau hauv cov xwm txheej ntawm citrate hlau, cov kab mob hydrogen sulfide tuaj yeem kuaj pom. Yog tias muaj cov kab mob uas tsim los ntawm hydrogen sulfide, nag lossis daus dub yuav raug tsim tawm ntawm cov ntsiav tshuaj. Tuliphenol xiav thiab thymphenol xiav. Ob lub tebchaw no yog qhov ntsuas pH. Crobenol xiav yog qhov ntsuas pH, thiab pH xim hloov ntau yam yog 3.8 (daj) ~ 5.4 (xiav-ntsuab). Muaj ob hom kev hloov xim: (1) Cov kua qaub ntau yog pH1.2 - 2.8, thiab liab los ntawm daj; (2) qhov ntau ntawm alkali yog 8.0 mus rau 9.6, los ntawm daj mus rau xiav.
4. TSA pancreatic soybean agar culture medium
Cov khoom xyaw TSA zoo ib yam li cov khoom noj khoom haus agar. Nyob rau hauv lub teb chaws tus qauv, nws yog feem ntau yog siv los ntsuam xyuas qhov kev xeem ntawm lub chav tsev tu (koog tsev kawm ntawv) ntawm kev lag luam kws tshuaj. Xaiv qhov ntsuas qhov ntsuas hauv cheeb tsam yuav tsum tau sim, qhib TSA ntsiav tshuaj, thiab npaj nws ntawm qhov ntsuas. Tshaj tawm hauv huab cua rau ntau tshaj 30 feeb thiab tsis txhob tos rau cov qauv, thiab tom qab ntawd cog qoob loo. Cov qib kev huv sib txawv yuav tsum muaj cov cheeb tsam sib txawv.
5. Mueller-Hinton agar
MH nruab nrab yog qhov nruab nrab microbial uas yog siv los kuaj xyuas cov kab mob mus rau cov tshuaj tiv thaiv kab mob. Nws yog ib qho kev xaiv nruab nrab, thiab feem ntau ntawm cov kab mob tuaj yeem loj hlob ntawm nws. Tsis tas li ntawd, cov hmoov txhuv nplej siab hauv cov khoom xyaw tuaj yeem nqus cov co toxins tawm los ntawm cov kab mob, yog li nws yuav tsis cuam tshuam rau cov txiaj ntsig ntawm kev ua haujlwm tshuaj tua kab mob. Cov khoom xyaw ntawm MH kab lis kev cai yog ntau xoob, uas yog qhov zoo rau kev sib kis ntawm cov tshuaj tua kab mob, yog li nws tuaj yeem pom pom pom kev loj hlob inhibitory bands.
Hauv Tuam Tshoj txoj kev lag luam kev noj qab haus huv, MH nruab nrab kuj yog siv rau kev kuaj tshuaj. Thaum ua qee cov kab mob tshwj xeeb rau kev kuaj tshuaj, xws li kab mob ntsws, 5 feem pua ntawm cov ntshav yaj thiab NAD tuaj yeem raug ntxiv rau qhov nruab nrab kom tau raws li cov kev xav tau ntawm kev noj haus sib txawv.
6.SS ua
SS agar feem ntau yog siv los ua Salmonella, xaiv kab lis kev cai ntawm Takagacia. Tiv thaiv cov kab mob Gram-positive thiab feem ntau ntawm cov kab mob colonic thiab Transforma, tab sis tsis cuam tshuam rau kev loj hlob ntawm Salmonella; sodium sulfate thiab hlau citrates yog siv los kuaj xyuas cov khoom ntawm hydrogen sulfide, ua rau qhov chaw ntawm cov cheeb tsam dub; nruab nrab liab; nruab nrab liab; nruab nrab liab; nruab nrab liab; nruab nrab liab; nruab nrab liab; nruab nrab liab; Rau qhov ntsuas pH, cov cheeb tsam ntawm fermented qab zib yog xim liab, thiab cov cheeb tsam ntawm cov suab thaj uas tsis yog-fermented tsis muaj xim. Salmonella yog ib qho tsis muaj xim pob tshab lossis cov chaw dub, thiab Toroshishae yog cov xim tsis muaj xim thiab pob tshab.
BKMAM khoom:
Petri tais
Inoculation voj voog
Cell kab lis kev cai phaj
Cell kab lis kev cai khob
Thiab lwm yam khoom siv (lab), txais tos sab laj nrog peb kom paub meej ntxiv:
