Tom qab ntxuav thiab tshuaj tua kab mob ua ntej siv, seb Petri phaj huv si los yog tsis muaj kev cuam tshuam zoo rau kev ua haujlwm, uas tuaj yeem cuam tshuam rau pH ntawm kab lis kev cai nruab nrab. Yog tias muaj qee yam tshuaj, nws yuav inhibit qhov kev loj hlob ntawm cov kab mob.
Petri cov tais diav tshiab yuav tsum tau ntxuav nrog dej kub ua ntej, tom qab ntawd tsau rau hauv cov tshuaj hydrochloric acid nrog cov feem ntau ntawm 1 feem pua lossis 2 feem pua rau ob peb teev kom tshem tawm cov tshuaj dawb alkaline, thiab tom qab ntawd ntxuav ob zaug nrog dej distilled.
Yog tias koj xav cog cov kab mob, ces siv lub zog siab (feem ntau 6.8 * 10 lub zog PA lub zog siab), sterilize ntawm 120 degree rau 30min, qhuav ntawm chav tsev kub, los yog sterilize nrog qhuav cua sov, uas yog, muab cov kab lis kev cai. tais hauv qhov cub thiab khaws cia ntawm li 120 degree rau 2h kom tua cov kab mob ntawm tes.
Tsuas yog sterilized Petri lauj kaub tais diav tuaj yeem raug inoculated thiab cultured.