Cryopreservation tube tseem hu ua strain preservation tube, magnetic hlaws preservation tube, thiab magnetic hlaws cryopreservation raj. Cov kev siv feem ntau rau kev khaws cov kab mob hauv microbiology kuaj muaj xws li mis nyuj, txoj kev glycerin, thiab txoj kev dav hlau inclined. Lub degree ntawm complexity txawv, thiab cov nyhuv txawv heev.
cryopreservation raj siv kho mob polypropylene (PP) raws li cov ntaub ntawv raw thiab yog cov khoom siv pov tseg pov tseg rau kev khaws cia cov qauv tshuaj lom neeg. Nyob rau hauv ib puag ncig gaseous ntawm cov kua nitrogen, nws tuaj yeem tiv taus qhov kub thiab txias mus txog 187 degrees. Qhov tshwj xeeb sab nraud kev sib hloov tsim kom tsis txhob muaj kev sib kis kab mob. Lub kaus mom lub raj yog nruab nrog lub silicone ncoo los tiv thaiv cov kua xau, thiab nws tuaj yeem ua kom nruj txawm tias nyob hauv qhov chaw cia txias, uas thaum kawg ua kom muaj kev nyab xeeb ntawm cov qauv hauv lub raj. Lub kaus mom yog nruab nrog ntau hom xim xim rau kev txheeb xyuas yooj yim. Qhov chaw cim dawb thiab meej meej ntawm lub raj lub cev yog yooj yim rau cov neeg siv los kos thiab ntsuas qhov ntim. Lub zog centrifugal tuaj yeem ncav cuag 17000g. Tag nrho cov cryotubes yog sterilized los ntawm gamma, tsis muaj DNase, RNase, thiab pyrogen.
cryopreservation tube tseem hu ua strain preservation tube, magnetic hlaws preservation tube, thiab magnetic hlaws cryopreservation raj. Cov kev siv feem ntau rau kev khaws cov kab mob hauv microbiology kuaj muaj xws li mis nyuj, txoj kev glycerin, thiab txoj kev dav hlau inclined. Lub degree ntawm complexity txawv, thiab cov nyhuv txawv heev. Tam sim no, feem ntau cov chaw soj nstuam hauv lub tebchaws ua cov kab mob khaws cia cov hlab ntsha los ntawm cov neeg sim lawv tus kheej, uas tsis tsuas yog ua rau kev ua haujlwm hnyav, tab sis kuj vim muaj kev txwv ntau yam, cov kab mob khaws cia cov nyhuv tsis yog ib txwm txaus siab.
Lub creation ntawm strain preservation raj ua rau cov no yooj yim heev.
cryopreservation tube yog ib lo lus dav dav rau kev lag luam lub npe strain preservation raj.
Lub raj cryopreservation ntawm strains yog tsim los ntawm peb qhov chaw: preservation solution, preservation tube thiab me me ceramic hlaws dai. Nws yog ib yam ntawm lub chaw soj nstuam khaws cia ntim rau strain preservation los yog hloov. Feem ntau, muaj 25 hom kab me me hauv cov raj khaws cia. Porcelain hlaws yog siv rau adsorption thiab khaws cia cov kab mob. Thaum siv, cov kab mob no tau nkag mus rau hauv lub raj khaws cia thiab muab tso rau hauv ib puag ncig ntawm -20 degree lossis -80 degree rau kev khaws cia mus ntev. (-20 degree tuaj yeem khaws cia rau ib xyoos, -80 degree tuaj yeem khaws cia rau ob xyoos).
Cia peb saib cov kauj ruam ntawm kev siv cryotubes
⑴ Xaiv cov kab lis kev cai tshiab los ntawm cov kab lis kev cai ntshiab ntawm cov kab mob los tsim cov kab mob tua kab mob nrog cov turbidity txog 3-4 Mai's turbidity thiab inoculate nws nyob rau hauv ib tug kab mob preservation raj.
⑵ Tighten lub raj khaws cia, tig nws nce thiab nqis 4-5 zaug kom emulsify cov kab mob, tsis tas yuav co.
⑶ Muab lub raj tso rau hauv lub tub yees kom khaws cia (-20 degree --70 degree )