Ordinary microplate
Cov khoom siv polymer siab tshaj plaws saum npoo kho tshuab thiab cov txheej txheem tsim khoom yog siv. Nws yog qhov tsim nyog rau kev nyab xeeb, txhim khu kev qha thiab muaj txiaj ntsig zoo hauv ELISA kev sim, thiab tuaj yeem siv rau kev tshuaj ntsuam xyuas enzyme-txuas immunosorbent: xws li: kev tiv thaiv kab mob, kev txheeb xyuas cov khoom hloov kho caj ces, thiab kev kuaj mob, thiab lwm yam.
Muaj peev xwm khi tau ELISA phaj
After the surface treatment of the target plate, the protein binding capacity is greatly enhanced, which can reach 300-400ng igG/cm², and the molecular weight of the main bound protein is >10kd ua. Kev siv hom ELISA phaj no tuaj yeem txhim kho rhiab heev, thiab tuaj yeem txo qhov concentration thiab ntau npaum li cas ntawm cov protein txheej. Qhov tsis zoo yog tias nws tsim cov tshuaj tiv thaiv tsis tshwj xeeb, antigen lossis cov tshuaj tiv thaiv tsis kam. Tom qab antigen los yog antigen txheej, non-ionic detergent tsis tuaj yeem kaw qhov chaw tsis muaj protein ntau, thiab cov protein yuav tsum tau siv los ua sealant.
Nruab nrab binding peev ELISA phaj
Passively binds to proteins via hydrophobic bonds on the surface, and is suitable as a solid-phase carrier for macromolecular proteins with a molecular weight of >20kd ua. Nws cov protein binding muaj peev xwm yog 200-300ng igG/cm², vim hais tias hom microplate no tsuas muaj macromolecule binding Features, haum raws li ib tug khoom-theem cov cab kuj rau unpurified antibodies los yog antigens, thiab yuav txo tau tej yam uas tsis yog-specific cross-reactions. Hom lub rooj tsavxwm no tuaj yeem siv los ua tus neeg saib xyuas thaiv nrog inert protein lossis tsis-ionic detergent