Dna Lobind Tubes

Dna lobind tubes, ntau dua rov qab muaj peev xwm lobind raj, ntau dua PCR efficiency

BKMAM dna lobind tubes tuaj yeem txo qhov pcr cov tshuaj tiv thaiv cov qauv poob thiab ua kom rov zoo dua ntawm cov nucleic acids muaj nuj nqis. Kev sib xyaw ua haujlwm zoo tshaj plaws thiab cov khoom siv zoo polypropylene, Nws tuaj yeem tiv taus qhov kub thiab txias ntawm 121 degree thiab tuaj yeem ua kom tsis muaj menyuam los ntawm qhov kub thiab txias, BKMAM dna lobind hlab yuav luag 100 feem pua ​​​​rov qab ntawm DNA thiab RNA molecules yam tsis xav tau txheej txheej. .

So kom ntseeg tau tias BKMAM dna lobind Tubes yog qhov zoo tshaj plaws thiab tsim nyog rau txhua daim ntawv thov suav nrog kev tshuaj xyuas kev tshawb fawb, microarray thiab tom ntej generating sequencing (NGS). BKMAM dna lobind Tubes tau dhau los ua cov kev ntsuas nruj thiab muaj ntawv pov thawj los ntawm lub chaw kuaj mob ywj pheej kom ntseeg tau tias lawv yog DNAse, RNase, pyrogen, tib neeg DNA thiab PCR inhibitors cov khoom pub dawb.

PCR technology Taw qhia los ntawm BKMAM:

Kuj tseem hu ua polymerase chain reaction. NDA yog ib qho txheej txheem rau kev nthuav dav thiab nthuav qhia cov kab ke DNA tshwj xeeb, uas ua rau ntau tus NDA tawg tawg nthuav dav. Ua ntej tuaj txog ntawm PCR thev naus laus zis, tib neeg siv cov qauv DNA ua tib zoo thaum tshawb nrhiav DNA, vim hais tias ua ntej PCR thev naus laus zis hauv vitro amplification, txhua tus qauv DNA muaj nuj nqis heev, thiab cov kws tshawb fawb yuav tsum siv me ntsis. Qhov tshwm sim ntawm PCR thev naus laus zis tau rhuav tshem qhov kev txwv no. Nws zoo ib yam li DNA replication nyob rau hauv lub cev, uas yog ib yam tshwj xeeb ntawm DNA replication nyob rau hauv lub cev, nyob qhov twg peb muaj peev xwm cais me ntsis ntawm DNA, peb muaj peev xwm amplify nws nrog PCR.

Cov txheej txheem PCR tuaj yeem muab faib ua peb theem: denaturation - annealing - extension.

Denaturation (90 degree -96} degree): Muab ob npaug rau stranded DNA template nyob rau hauv tshav kub, hydrogen daim ntawv cog lus so, tsim ib leeg stranded DNA.

Annealing: (60 degree -65} degree): qhov system kub txo thiab cov primer khi rau DNA template los tsim ib lub zos ob strand.

Elongation: (70 degree -75 degree): Nyob rau hauv qhov kev txiav txim ntawm Taq enzyme (kwv yees li 72 degree, qhov kev ua haujlwm zoo tshaj plaws), siv dNTP ua raw khoom, pib los ntawm 3 'kawg ntawm primer thiab txuas ntxiv ntawm 5' mus rau 3 'kawg, DNA saw txuas ntxiv rau cov qauv yog synthesized.

Raws li pom nyob rau hauv daim duab hauv qab no, tom qab ib tug denaturing annealing txheej txheem, tus thawj DNA ob strand tau hloov mus rau hauv ob strands. Tom qab 2, 4; Los ntawm kev sib piv, kaum ob lub voj voog tuaj yeem tsim ntau tus lej DNA zoo ib yam.

Kev ntsuam xyuas tom qab:

Qhov no yog cov txheej txheem ntawm cov pa PCR. Tom qab qhov kev nthuav qhia tiav lawm, peb yuav tsum tau kuaj cov DNA amplified, thiab cov txheej txheem PCR feem ntau tsuas yog siv gel electrophoresis. Lub tshuab electrophoresis yuav tsis qhia ntawm no. Nws yog ib qho cuab yeej uas cais DNA raws li qhov hnyav molecular ntawm DNA, thiaj li txheeb xyuas thiab ntxuav DNA. Gel electrophoresis tuaj yeem txiav txim siab seb qhov loj ntawm qhov loj ntawm DNA tawg puas yog raws li qhov loj ntawm lub hom phiaj band, thiaj li paub seb cov khoom xav tau puas tau. Xyuas seb puas muaj heterozygous bands thiab txiav txim seb lub amplification puas muaj tseeb. Saib seb puas muaj primer dimer thiab txiav txim qhov tsim ntawm primer thiab lwm yam.

BKMAM muab cov khoom siv sib xws rau cov txheej txheem PCR:

PCR raj / yim strip raj / daim hlau:

100 feem pua ​​​​Kev kho mob qib Polypropylene PP cov khoom siv los xyuas kom meej tias nyob rau hauv cov txheej txheem ntawm denaturation ntawm qhov kub thiab txias, cov khoom yuav tsis tsim deformation puas thiab lwm yam teeb meem;

Nyias-phab ntsa tsim, yooj yim rau kev kub ceev ceev hauv cov txheej txheem cua sov, txhim kho PCR efficiency;

Kev sib khi zoo heev, txawm tias me me ntawm PCR system yuav tsis evaporate vim qhov kub thiab txias, txo qhov poob ntawm cov tshuaj tiv thaiv ntim;

Tsis muaj enzyme thiab tsis muaj pyrogens, kom ntseeg tau tias koj cov qauv DNA yuav tsis raug degraded vim muaj kab mob;

Xws li cov pob tshab ob xim, xim dawb hauv qPCR kev sim kom txo tau qhov cuam tshuam ntawm fluorescence, qhov teeb meem fluorescence tshaj plaws thiab raug;

Plate consumables muaj nyob rau hauv ntau hom hemline kom yooj yim txuam nyob rau hauv txawv PCR tshuab los yog automation daim ntaub ntawv.

PCR sealing phaj membrane:

Zoo sealing kom txo qhov poob ntawm cov qauv;

Xws li viscous sealing zaj duab xis thiab siab rhiab sealing zaj duab xis ob hom kev xaiv;

Lub siab pob tshab ntawm lub siab-rhiab zaj duab xis ua rau nws yooj yim mus ntes cov teeb liab fluorescence txawm tias koj siv nws hauv qPCR.

Thov xaiv qhov tsim nyog dna lobind raj los ntawm peb cov PCR raj SPEC daim ntawv teev npe:

Koj qhov kev txhim khu kev qha PCR raj xa khoom, 10 lub chaw tsim khoom zoo siab PCR raj hauv Suav teb.

Ethylene oxide sterilized dna lobind raj nrog ISO9001: 2015, FDA & CE ntawv pov thawj

26, 000 square meters ntawm 100, 000- theem purification Rhiav, thiab 1, 000 square meters ntawm high-precision pwm R&D thiab ntau lawm rhiav, BKMAM muaj zog txaus muaj peev xwm ua tau raws li koj xav tau rau lub ntsiab PCR Tubes.we kuj muaj peev xwm muab PCR sawb raj pwm tsim kev pab cuam, peb pwm tsim thiab ua cov nqi qis, thiab cov lus teb ceev ceev rau cov neeg siv khoom xav tau, Pab koj ceev ceev ntawm kev lag luam tshiab ntawm cov khoom siv.

Ua tsaug vam tias yuav tsim kom muaj kev sib raug zoo ua lag luam nrog koj lub tuam txhab hwm.

Michael Peng

Changde BKMAM Biotechnology Co., Ltd

Chaw Nyob: Lub Tsev 1, East Of New Material Industial Zone, Linli Economic Development Zone, Changde, 415200 Hunan

Tel: plus 86-17363688083

Mob: plus 8613450479355(whatsapp&wechat)

Skype: peng.michael2011

www.bikeman-bio.com

www.bkmio.com

https://hnbikeman.en.alibaba.com

Email: michael@bkmbio.com