Cell Culture kho daim hlau

Cell kab lis kev cai kho daim hlau, TC kho, DNAse, RNase, pyrogen thiab hlau dawb kab lis kev cai daim hlau

Koj qhov txhim khu kev qha cell kab lis kev cai kho plateds tsum, sab saum toj 10 chaw tsim tshuaj paus ntawm zoo cell kab lis kev cai daim hlau nyob rau hauv Suav teb.

High quality thiab high yield cell kab lis kev cai kho daim hlau los ntawm Tuam Tshoj

BKMAM cell kab lis kev cai daim hlau tswj qhov system, khoom warranty rau 3 xyoos

Peb siv ntau hom kab lis kev cai zoo ntawm tes los pab ua kom muaj kev noj qab haus huv, kev loj hlob ntawm tes.

Polystyrene cell kab lis kev cai daim hlau muaj nyob rau hauv ntau tshaj 100 hom thiab nto ua ke los pab txhawb kev noj qab haus huv ntawm tes thiab muab cov txiaj ntsig zoo rau koj cov kev tshawb fawb.

High quality thiab high yield cell kab lis kev cai kho daim hlau

Peb yog cov kws tshaj lij kab lis kev cai phaj chaw tsim khoom hauv Suav teb, peb tuaj yeem muab cov kab lis kev cai ntawm tes nrog ntau yam kev kho mob saum npoo

Nrhiav cov kab lis kev cai ntawm tes uas koj xav tau raws li qhov sib txawv ntawm cov kab lis kev cai ntawm tes:

Txhawm rau pab ua kom pom cov txiaj ntsig zoo rau cov kab lis kev cai sib txawv ntawm tes thiab kev tshuaj ntsuam xyuas ntawm tes, peb muab ntau hom kab lis kev cai ntawm tes nrog ntau qhov chaw, zoo hauv qab cov duab thiab xim. Cia peb pab koj xaiv los ntawm cov kab lis kev cai ntawm tes uas tau hloov kho kom tau raws li qhov xav tau ntawm koj cov hom cell tshwj xeeb.

1. BKMAM cell kab lis kev cai kho daim hlau yog tsim rau cov hlwb adherent

Txheem cov ntaub so ntswg kab lis kev cai (TC) nto kho mob ua rau polystyrene nto ntau hydrophilic, muab siab tshaj plaws adhesion rau ntau hom cell.

2. Cell kab lis kev cai daim hlau tsis kho rau kev ncua kev lis kev cai los yog niaj hnub tsom xam:

High-quality, light-transmitting, non-reconstituted polystyrene nrog ib tug hydrophobic nto yog zoo tagnrho rau ncua kev kawm cell kab lis kev cai thiab yog sib npaug pab tau nyob rau hauv ntau yam biochemical binding assays.

3. BKMAM cell kab lis kev cai daim hlau rau culturing xav tau hlwb

Lub zog tshwj xeeb-kho qhov chaw, ua ke nrog cov txheej txheem nruab nrab ntxiv nrog ROCK inhibitor, txhawb kev txuas thiab nthuav dav ntawm tib neeg pluripotent qia hlwb.

4. BKMAM cell kab lis kev cai daim hlau rau spheroid cell kab lis kev cai

Txhawb nqa cov kab lis kev cai spheroid. Cells loj hlob thiab aggregate nrog me ntsis txuas mus rau kab lis kev cai hlab ntsha. Haum rau kab lis kev cai spheroid, kab lis kev cai nqaij thiab 3D kab lis kev cai.

5. BKMAM cell kab lis kev cai daim hlau rau adherent kab lis kev cai thiab sau cov hlwb uas tsis tas yuav trypsin

Ua kom cov qoob loo ntawm cov hlwb hauv ib lub xov tooj raug tshem tawm lossis cov txheej txheem ntawm tes txuas ntxiv los ntawm kev txo qhov kub, ua kom muaj cov qauv 3D cov ntaub so ntswg yam tsis muaj cov khoom siv hauv vitro scaffolding.

6. Poly-D-Lysine thiab Collagen BKMAM cell kab lis kev cai daim hlau rau thawj thiab xav tau cov hlwb

ECM-coated nto mimic nyob rau hauv vivo kev loj hlob tej yam kev mob thiab yog zoo tagnrho rau culturing hlwb uas nyuaj rau loj hlob nyob rau hauv cov ntaub so ntswg kab lis kev cai nto. Collagen I yog tsiaj keeb kwm, thaum PDL yog hluavtaws tag nrho.

Ua tiav qhov loj ntawm sab saum toj zoo cell kab lis kev cai daim hlau:

Hom Cell kab lis kev cai daim hlau:

Xaiv los ntawm cov duab hauv qab

Raws li cov duab hauv qab, nws tuaj yeem muab faib ua lub tiaj tiaj thiab puag ncig hauv qab (U hom thiab V cell kab lis kev cai phaj hom)

Txhua yam ntawm cov hlwb hauv qab tuaj yeem siv tau, tab sis thaum muaj pes tsawg lub hlwb me me, xws li cloning, siv lub 96-zoo tiaj-hauv qab. Tsis tas li ntawd, thaum ua MTT thiab lwm yam kev sim, ob qho tib si adherent thiab suspended hlwb feem ntau flat-bottomed. Raws li rau U lossis V-shaped daim hlau, lawv feem ntau yog siv thaum qee qhov tshwj xeeb xav tau. Piv txwv li, hauv kev tiv thaiv kab mob, thaum ob hom lymphocytes sib xyaw ua ke thiab kab lis kev cai, lawv yuav tsum muaj kev sib cuag nrog ib leeg rau kev txhawb nqa. Yog li ntawd, U-zoo li daim hlau feem ntau yuav tsum tau, vim hais tias cov hlwb yuav sib sau ua ke nyob rau hauv ib tug me me npaum li cas ntawm cov nyhuv ntawm lub ntiajteb txawj nqus. V-shaped daim hlau tsis tshua muaj txiaj ntsig. Feem ntau, hauv kev sim tua cov xov tooj ntawm tes, txhawm rau ua kom lub hom phiaj zoo hauv kev sib raug zoo, V-shaped daim hlau feem ntau siv, tab sis U-puab daim hlau kuj tseem siv tau los hloov U-puab daim hlau (tom qab ntxiv cov hlwb, qis-ceev centrifugation. ). Yog tias koj xav kom loj hlob hlwb, koj feem ntau xaiv cov hauv qab tiaj tus. Tsis tas li ntawd, koj yuav tsum tau them nyiaj tshwj xeeb rau cov khoom siv. Nws zoo nkaus li koj tsis tau hnov ​​​​txog cov hauv qab hauv qab rau cov hlwb loj hlob. Lub puag ncig hauv qab feem ntau yog siv rau kev tsom xam, tshuaj lom neeg cov tshuaj tiv thaiv, los yog khaws cia cov qauv, vim hais tias lub puag ncig hauv qab yog zoo dua rau nqus cov kua huv si, thiab yog tias koj siv lub hauv qab tiaj tus, nws tsis yooj yim rau nqus. Txawm li cas los xij, yog tias koj xav ntsuas qhov nqus, koj yuav tsum yuav lub hauv qab tiaj tus. Feem ntau cov kab lis kev cai ntawm tes siv cov kab lis kev cai tiaj tus hauv qab kab lis kev cai, uas yooj yim los soj ntsuam hauv qab lub tshuab ntsuas, muaj thaj chaw hauv qab kom meej, muaj cov kab lis kev cai ntawm cov kua dej sib txawv, thiab yooj yim rau kev tshawb nrhiav MTT.

Cov kab lis kev cai puag ncig-hauv qab feem ntau yog siv rau kev sim ntawm isotope incorporation, uas yuav tsum tau cov kab lis kev cai ntawm cov hlwb yuav tsum tau sau nrog lub cell harvester.

Xaiv los ntawm cov khoom siv

Nyob ntawm cov khoom siv, muaj Terasaki daim hlau thiab cov kab lis kev cai ntawm tes zoo tib yam. Cov kev xaiv tshwj xeeb yog nyob ntawm hom kab lis kev cai, qhov ntim kab lis kev cai xav tau thiab cov hom phiaj sib txawv.

Lub cell kab lis kev cai phaj tuaj yeem muab faib ua lub tiaj tiaj thiab puag ncig hauv qab (U-puab thiab V-shaped) raws li cov duab hauv qab; Terasaki daim hlau thiab cov kab lis kev cai ntawm tes zoo tib yam.

Yuav ua li cas xaiv cov kab lis kev cai ntawm tes kom raug

Lub cell kab lis kev cai phaj tuaj yeem muab faib ua lub tiaj tiaj thiab puag ncig hauv qab (U-puab thiab V-shaped) raws li cov duab hauv qab; Terasaki daim hlau thiab cov kab lis kev cai ntawm tes zoo tib yam.

Qhov sib txawv thiab kev xaiv ntawm lub tiaj tiaj hauv qab thiab puag ncig hauv qab (U-shaped thiab V-shaped) kab lis kev cai daim hlau

Cov duab sib txawv ntawm kab lis kev cai ua haujlwm sib txawv. Rau culturing hlwb, ib tug ca hauv qab feem ntau yog siv, uas yog yooj yim rau kev soj ntsuam nyob rau hauv lub microscope, muaj ib tug meej hauv qab cheeb tsam, thiab qhov siab ntawm lub cell kab lis kev cai kua yog zoo ib yam. Yog li ntawd, thaum ua MTT thiab lwm yam kev sim, txawm tias nws yog adherent lossis tshem tawm cov hlwb, feem ntau siv lub phaj hauv qab. Nco ntsoov siv lub tiaj-hauv qab kab lis kev cai phaj rau kev ntsuas absorbance. Ua tib zoo saib xyuas cov khoom siv, daim ntawv lo "Tissue Culture (TC) Treated" yog rau cov hlwb loj hlob.

U-shaped lossis V-shaped daim hlau feem ntau yog siv thaum qee qhov tshwj xeeb xav tau. Piv txwv li, hauv kev tiv thaiv kab mob, thaum ob hom lymphocytes sib xyaw ua ke thiab kab lis kev cai, lawv yuav tsum sib cuag thiab txhawb nqa ib leeg. Lub sijhawm no, feem ntau siv U-shaped phaj, vim hais tias cov hlwb yuav sib sau ua ke hauv ib qho me me vim qhov cuam tshuam ntawm lub ntiajteb txawj nqus. Round-hauv qab kab lis kev cai daim hlau kuj tseem siv rau isotope incorporation thwmsim uas yuav tsum tau siv cov cell harvesters los sau cell kab lis kev cai, xws li "sib xyaw lymphocyte cultures". V-plates feem ntau yog siv rau kev tua cell thiab kev sim tshuaj tiv thaiv kab mob hemagglutination. Qhov kev sim ntawm kev tua cell no kuj tuaj yeem hloov tau los ntawm U-phaj (tom qab ntxiv cov hlwb, centrifuge ntawm qis ceev).

Qhov txawv ntawm Terasaki phaj thiab zoo tib yam cell kab lis kev cai phaj

Terasaki phaj feem ntau yog siv rau kev tshawb fawb crystallographic, thiab cov khoom tsim yog yooj yim rau kev soj ntsuam siv lead ua thiab kev tsom xam. Muaj ob txoj hauv kev zaum thiab tuav poob, thiab cov duab thiab cov qauv ntawm cov khoom siv los ntawm ob txoj kev kuj txawv. Xaiv cov chav kawm siv lead ua polymer rau cov khoom siv, cov ntaub ntawv tshwj xeeb muaj txiaj ntsig zoo los saib xyuas cov qauv siv lead ua.

Lub cell kab lis kev cai phaj yog tsuas yog ua los ntawm PS cov khoom, thiab cov khoom yog kho sufface, uas yog yooj yim rau cell adherent kev loj hlob thiab extension. Tau kawg, kuj tseem muaj cov ntaub ntawv loj hlob rau cov hlwb planktonic, nrog rau cov ntawv khi qis

Feem ntau siv zoo hauv qab cheeb tsam ntawm cov kab lis kev cai sib txawv thiab pom zoo ua kua ntim

Cov kua dej ntawm cov kab lis kev cai nruab nrab ntxiv rau cov phiaj xwm sib txawv yuav tsum tsis txhob sib sib zog nqus, feem ntau nyob rau hauv thaj tsam ntawm 2 ~ 3 hli, thiab qhov tsim nyog cov kua ntxiv rau txhua kab lis kev cai zoo tuaj yeem xam raws li thaj tsam hauv qab ntawm cov qhov dej sib txawv ( xa mus rau cov lus hauv qab no). Yog tias cov kua ntxiv ntau dhau lawm, nws yuav cuam tshuam rau kev sib pauv ntawm cov pa roj (oxygen), thiab nws yooj yim kom dhau thiab ua rau muaj kuab paug thaum lub sijhawm txav mus los. Qhov tshwj xeeb ceev ntawm cov cell yuav tsum tau ntxiv yog nyob ntawm lub hom phiaj ntawm qhov kev sim.

Ib-stop Laboratory Consumables tsum!

Peb tab tom nrhiav rau cov neeg ua haujlwm ncaj ncees thoob plaws lub ntiaj teb thiab ua siab dawb tos ntsoov yuav koom tes nrog koj.

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