ELISA 96 zoo phaj, tag nrho cov dub thiab tag nrho cov dawb ELISA phaj, tiaj hauv qab, luminescent phaj, Independent sterilization pob, TC adherence kev kho mob, sterile elisa daim hlau
Sterile elisa 96 phaj, ntsuas Fluorescence Luminescent phaj tsom xam phaj Tshem tau siab adsorption TC kev kho mob ywj pheej ntim dawb hauv qab pob tshab
Kev faib tawm: 16 qhov dej, 48 qhov dej thiab 96 qhov dej
Hom Tshuaj: BKMAM Biotechnology
Khoom siv: Polystyrene
Xim: dawb elisa phaj, dub elisa phaj
Ntim: 1 pc / hnab sterile ywj siab ntim
Cov khoom siv: kev coj noj coj ua dav dav, kab lis kev cai kab mob, kab yug tsiaj, thiab lwm yam.
ELISA PLATE Hauv enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA), purity, concentration thiab ratio of antigen, antibody, labeled antibody or antigen koom nrog cov tshuaj tiv thaiv kab mob; Tsis yog hom, concentration thiab ion Cov xwm txheej xws li lub zog, pH thiab cov tshuaj tiv thaiv kub thiab lub sijhawm ua lub luag haujlwm tseem ceeb.
Cov khoom siv-theem polystyrene (Polystyrene) nto raws li tus neeg nqa khoom kuj tseem ua lub luag haujlwm tseem ceeb hauv adsorption ntawm antigens, antibodies lossis antigen-antibody complexes.
Antigens, cov tshuaj tiv thaiv kab mob, thiab lwm yam biomolecules yog adsorbed rau hauv cov cab kuj los ntawm ntau yam txheej txheem, suav nrog kev ua haujlwm tsis zoo los ntawm hydrophobic bonds, dej / ionic bonds, covalent binding los ntawm kev qhia txog lwm pawg reactive xws li amino thiab carbon pawg, thiab kev hloov kho saum npoo. . Hydrophilic daim ntawv cog lus tom qab hloov kho kho.
Hom Elisa daim hlau feem ntau yog xaiv raws li tus nyeem ntawv microplate. Nws muab faib ua detachable thiab non-detachable. Rau cov uas tsis yog-detachable, cov slats ntawm ib tug tag nrho lub rooj tsavxwm yog txuas ua ke, ces lub detachable txhais tau hais tias cov slats ntawm lub rooj tsavxwm yog sib cais, thiab cov slats sib cais. Muaj 12 qhov thiab 8 qhov. Feem ntau, detachable enzyme-labeled daim hlau yog siv ntau dua. Yog tias koj tau yuav qee cov phiaj xwm ua ntej, koj tuaj yeem yuav ib co enzyme-labeled slats. Txawm hais tias cov microplates ua los ntawm cov tuam txhab sib txawv zoo ib yam ntawm tag nrho, qee cov ntsiab lus me me yuav txawv, xws li cov qauv, thiab lwm yam. Qhov no yog vim nws yuav tsum tau siv nrog cov sib txawv microplate nyeem. Kuj xav txog seb koj tus nyeem ntawv microplate yuav zoo li cas thaum xaiv yuav microplate. Txawm li cas los xij, lawv feem ntau tsim nyog, thiab tsuas yog tus kheej yuav txawv. Vim tias cov khoom siv ntawm lub microplate feem ntau yog polystyrene (PS), thiab cov tshuaj ruaj khov ntawm polystyrene tsis zoo, nws tuaj yeem yaj los ntawm ntau yam organic solvents (xws li cov tshuaj tsw qab hydrocarbons, halogenated hydrocarbons, thiab lwm yam), thiab yuav raug corroded. muaj zog acids thiab alkalis, Tsis tiv taus roj, yooj yim hloov xim tom qab raug ultraviolet lub teeb, yog li nco ntsoov xyuam xim rau cov no thaum siv.
High binding ELISA phaj
The protein binding capacity of the ELISA plate after surface TC treatment is greatly enhanced, reaching 300-400ng 1gG/cm2, and the molecular weight of the main binding protein is >10kd ua. Kev siv hom ELISA phaj no tuaj yeem txhim kho qhov rhiab heev, thiab tuaj yeem txo qhov concentration thiab ntau npaum li cas ntawm cov protein coated, tab sis qhov tsis zoo yog tias nws yooj yim los tsim cov tshuaj tiv thaiv tshwj xeeb. Tom qab cov tshuaj tiv thaiv kab mob los yog cov tshuaj tiv thaiv kab mob, cov tshuaj tsis-ionic tsis tuaj yeem thaiv qhov chaw tsis muaj protein ntau, thiab yuav tsum muaj protein ntau raws li tus neeg saib xyuas thaiv.
Nruab nrab khi ELISA phaj
Lub phaj ELISA tau dhau los ua ke rau dawb ntawm cov khoom siv hydrophobic, thiab yog qhov tsim nyog raws li cov txheej txheem thauj khoom rau macromolecular proteins nrog qhov hnyav molecular ntawm 20D, thiab nws cov protein khi muaj peev xwm yog 200-300 ng 1gG / cm2. Txij li hom ELISA phaj no tsuas yog khi rau macromolecules, nws yog qhov tsim nyog rau kev siv los ua cov khoom thauj khoom rau cov tshuaj tiv thaiv tsis huv lossis cov tshuaj tiv thaiv kab mob, uas tuaj yeem txo qhov tsis muaj qhov cuam tshuam tshwj xeeb. Hom phaj no tuaj yeem siv los thaiv kev daws nrog inert protein lossis tsis-ionic detergent
Aminase phaj
Tom qab kev hloov kho saum npoo, daim ntawv cawv cawv muaj cov pab pawg amino zoo, thiab nws daim ntawv cog lus hydrophobic yog hloov los ntawm kev tua dej. Hom ELISA phaj no yog tsim los ua cov khoom thauj khoom rau cov protein me me. Siv qhov tsis zoo thiab pH, nws qhov chaw tuaj yeem khi tsis zoo rau cov khoom me me ntawm cov ntawv cog lus ionic. Vim muaj cov khoom siv hydrophilic ntawm nws qhov chaw thiab nws lub peev xwm los khi cov khoom sib txuas los ntawm lwm cov neeg sib txuas sib txuas, nws tuaj yeem siv los hloov cov protein molecules yaj hauv cov tshuaj ntxuav tes xws li Triton-100 thiab Tween20. Qhov tsis zoo ntawm hom phaj no yog vim qhov txo qis hydrophobicity, qee cov protein molecules tsis tuaj yeem khi; Tsis tas li ntawd, qhov saum npoo yuav tsum tau thaiv kom zoo. Vim yog cov khoom siv hydrophilic thiab covalent nto, cov tshuaj thaiv kev siv yuav tsum muaj peev xwm cuam tshuam nrog cov amino pawg uas tsis yog reactive thiab txhua pab pawg ua haujlwm hauv crosslinker xaiv.
Protein Adsorption Determination ntawm ELISA Phaj
Daim hlau dawb polystyrene rau ELISA tuaj yeem coated nrog kev paub txog cov kua protein. Tom qab txheej txheej, cov protein concentration ntawm cov tshuaj hauv qhov dej tau ntsuas. Tus nqi ntawm cov protein nyob hauv qhov dej ua ntej txheej tshem tawm cov protein tom qab txheej yog cov protein adsorbed ntawm ELISA phaj. Ua li no, lub peev xwm adsorption ntawm ELISA phaj tuaj yeem paub.
Ib-stop Laboratory Consumables tsum!
Peb tab tom nrhiav rau cov neeg ua haujlwm ncaj ncees thoob plaws lub ntiaj teb thiab ua siab dawb tos ntsoov yuav koom tes nrog koj.
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